Journal
INTEGRATIVE MEDICINE RESEARCH
Volume 5, Issue 2, Pages 140-150Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imr.2016.04.005
Keywords
HPLC-DAD; LC/MS; simultaneous determination; validation; Yijin-tang
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Funding
- Korea Institute of Oriental Medicine, Ministry of Science, ICT, and Future Planning, Republic of Korea [K15280]
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Background: Yijin-tang (YJ) has been used traditionally for the treatment of cardiovascular conditions, nausea, vomiting, gastroduodenal ulcers, and chronic gastritis. In this study, a simple and sensitive high-performance liquid chromatography (HPLC) method was developed for the quantitation of nine bioactive compounds in YJ: homogentisic acid, liquiritin, naringin, hesperidin, neohesperidin, liquiritigenin, glycyrrhizin, 6-gingerol, and pachymic acid. Methods: Chromatographic separation of the analytes was achieved on an RS Tech C-18 column (4.6 mm x 250 mm, 5 mu m) using a mobile phase composed of water containing 0.1% (v/v) trifluoroacetic acid (TFA) and acetonitrile with a gradient elution at a flow rate of 1.0 mL/min. Results: Calibration curves for all analytes showed good linearity (R-2 >= 0.9995). Lower limits of detection and lower limits of quantification were in the ranges of 0.03-0.17 mu g/mL and 0.09-0.43 mu g/mL, respectively. Relative standard deviations (RSDs; %) for intra- and interday assays were < 3%. The recovery of components ranged from 98.09% to 103.78%, with RSDs (%) values ranging from 0.10% to 2.59%. Conclusion: This validated HPLC method was applied to qualitative and quantitative analyses of nine bioactive compounds in YJ and fermented YJ, and may be a useful tool for the quality control of YJ. (C) 2016 Korea Institute of Oriental Medicine. Published by Elsevier.
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