4.7 Article

Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Journal

EMERGING INFECTIOUS DISEASES
Volume 22, Issue 8, Pages 1448-1451

Publisher

CENTERS DISEASE CONTROL
DOI: 10.3201/eid2208.160270

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Funding

  1. United States Army
  2. Edgewood Chemical Biological Center Innovative Project [219]
  3. US National Academy of Science
  4. US Defense Threat Reduction Agency as a National Research Council fellow
  5. prime contract of Battelle Memorial Institute, Columbus, Ohio
  6. US National Institute of Allergy and Infectious Diseases [HHSN272200700016I]

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Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization.

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