Journal
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
Volume 149, Issue 5, Pages 412-417Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/ajcp/aqy008
Keywords
Glioblastoma; microRNA; MGMT; Methylation; Prognosis
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Funding
- Ministry of Science and Technology, Taiwan [MOST 104-2320-B-075-003, MOST 104-2320-B-075-004]
- Taipei Veterans General Hospital [V105C-185, V105C-187, V106C-037]
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To evaluate the prognostic values of microRNAs (miRNAs) in glioblastoma, and to see if there is an association between miRNAs and MGMT promoter methylation status. We collected paraffin blocks from resection specimens from 114 glioblastoma patients who had received temozolomide treatment and radiotherapy. Real-time quantitative PCR was performed to determine the expression levels of five miRNAs. Upregulation of miR 125b-5p, miR 181d-3p, miR 221-3p, miR-222-3p, and miR 224-5p was observed in 13.2%, 5.3%, 12.3%, 32.5%, and 78.9% of the cases, respectively. The expression level of miRNAs was not significantly different in tumors with MGMT promoter methylation vs tumors without such methylation. Upregulation of miR 125b-5p, miR 181d-3p, or miR 221-3p was significantly associated with shorter survival in MGMT-unmethylated glioblastoma patients. miR 125b-5p, miR 181d-3p, and miR 221-3p are useful in predicting poor prognosis in patients with MGMT-unmethylated glioblastomas.
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