Journal
AMERICAN JOURNAL OF CHINESE MEDICINE
Volume 46, Issue 3, Pages 651-671Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S0192415X18500349
Keywords
Acute Lung Injury; Asatone; Lipopolysaccharide; Antioxidative Enzymes; NF-kappa B; Mitogen-Activated Protein Kinases; Sepsis
Funding
- National Science Council [MOST 105-2320-B-039-046, MOST 106-2320-B-039-045]
- China Medical University (CMU) [ASIA104-CMUH-06, CMU105-ASIA-23]
- Asis University [105-ASIA-05, 105-ASIA-06, 105-ASIA-07]
- Tzu Chi University [TCCCF-P-103003]
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Asatone is an active component extracted from the Chinese herb Radix et Rhizoma Asari. Our preliminary studies have indicated that asatone has an anti-inflammatory effect on RAW 264.7 culture cells challenged with lipopolysaccharide (LPS). Acute lung injury (ALI) has high morbidity and mortality rates due to the onset of serious lung inflammation and edema. Whether asatone prevents ALI LPS-induced requires further investigation. In vitro studies revealed that asatone at concentrations of 2.5-20 mu g/mL drastically prevented cytotoxicity and concentration-dependently reduced NO production in the LPS-challenged macrophages. In an in vivo study, the intratracheal administration of LPS increased the lung wet/dry ratio, myeloperoxidase activity, total cell counts, white blood cell counts, NO, iNOS, COX, TNF-alpha, IL-1 beta, and IL-6 in the bronchoalveolar lavage fluid as well as mitogen-activated protein kinases in the lung tissues. Pretreatment with asatone could reverse all of these effects. Asatone markedly reduced the levels of TNF-alpha and IL-beta in the lung and liver,
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