Journal
EXPERIMENTAL DERMATOLOGY
Volume 24, Issue 2, Pages 157-159Publisher
WILEY-BLACKWELL
DOI: 10.1111/exd.12594
Keywords
analysis; histochemistry; immunofluorescence; immunohistochemistry; method
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Funding
- NIAMS [P30-AR057217]
- National Space Biomedical Research Institute (NSBRI) through NASA [NCC 9-58]
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Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open-source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation-induced tanning response of Yucatan mini-pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples.
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