Journal
SUSTAINABLE ENVIRONMENT RESEARCH
Volume 26, Issue 5, Pages 235-242Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.serj.2016.05.001
Keywords
Biohydrogen; Central composite design (CCD); Response surface methodology (RSM); Sugarcane bagasse; Hydrolysate
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Funding
- Research Group for Development of Microbial Hydrogen Production Process from Biomass
- Higher Education Research promotion and National Research University Projects for Thailand office of the Higher Education Commission
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The aim of this study is to determine the optimum condition for biohydrogen production from sugarcane bagasse (SCB) hydrolysate using a central composite design and response surface methodology (RSM). SCB was hydrolyzed with 0.5% (v/v) sulfuric acid at 121 degrees C, 0.15 MPa for 60 min in an autoclave at a solid to liquid ratio of 1: 15 (g:mL). Heat-treated bacterium obtained from a hydrogen producing fermentor was used as the inoculum. The interaction of three factors, i.e., substrate concentration, substrate: buffer ratio and inoculum: substrate ratio on hydrogen production potential (P) were investigated. The results indicated that the substrate concentration, substrate: buffer ratio and inoculum: substrate ratio had a significant influence effect on P. An optimal condition was found at substrate concentration of 22.77 gtotal sugar L-1, 4.31 substrate: buffer ratio, and 0.31 inoculum: substrate ratio resulted in a maximum P of 6980 mL H-2 L-1. The confirmation experiment results indicated that optimum P was statistically significant, from the predicted value obtained by RSM which suggests that RSM could be efficiently used to optimize a biohydrogen production from SCB hydrolysate using mixed cultures. These results indicates that the SCB hemicellulose hydrolysate is suitable as a fermentation media for producing biohydrogen. This approach will add value to SCB by converting agricultural waste into a safe and clean form of energy. (C) 2016 Chinese Institute of Environmental Engineering, Taiwan. Production and hosting by Elsevier B.V.
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