Journal
BIOFABRICATION
Volume 8, Issue 3, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/8/3/035014
Keywords
collagen; hydrobead; extracellular matrix; remodeling
Funding
- Korea Institute of Radiological and Medical Sciences - Ministry of Science, ICT & Future Planning [1711021925]
- National Research Foundation of Korea [2014M3A7B4052193]
- Human Resources Development of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Korea government Ministry of Trade, Industry Energy [20144010200770]
- Grants-in-Aid for Scientific Research [16H03173] Funding Source: KAKEN
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We presented a new quantitative analysis for cell and extracellular matrix (ECM) interactions, using cell-coated ECM hydrogel microbeads (hydrobeads) made of type I collagen. The hydrobeads can carry cells as three-dimensional spheroidal forms with an ECM inside, facilitating a direct interaction between the cells and ECM. The cells on hydrobeads do not have a hypoxic core, which opens the possibility for using as a cell microcarrier for bottom-up tissue reconstitution. This technique can utilize various types of cells, even MDA-MB-231 cells, which have weak cell-cell interactions and do not form spheroids in conventional spheroid culture methods. Morphological indices of the cell-coated hydrobead visually present cell-ECM interactions in a quantitative manner.
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