Journal
FEMS MICROBIOLOGY LETTERS
Volume 363, Issue 13, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnw117
Keywords
lactic acid bacteria; lactobacilli; Lactobacillus gasseri; in vivo-inducible system; inflammation; caries
Categories
Funding
- DIM Maladies Infectieuses, Parasitaires et Nosocomiales Emergentes (Malinf)
- Egypt-France Scientific and Technological Cooperation Program (Imhotep) [EGY/FR001, 98]
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Food-grade lactic acid bacteria, such as lactobacilli, represent good candidates for the development of mucosal vectors. Indeed, they are generally recognized as safe microorganisms and some strains display beneficial effects (probiotics). In this study, we described a new lactobacilli in vivo expression (LIVE) system for the production and delivery of therapeutic molecules at mucosal surfaces. The versatility and functionality of this system was successfully validated in several lactobacilli species; furthermore, we assessed in vivo LIVE system in two different mouse models of human pathologies: (i) a model of therapy against intestinal inflammation (inflammatory bowel diseases) and (ii) a model of vaccination against dental caries. We demonstrated that Lactobacillus gasseri expressing the anti-inflammatory cytokine IL-10 under LIVE system efficiently delivered the recombinant protein at mucosal surfaces and display anti-inflammatory effects. In the vaccination model against caries, LIVE system allowed the heterologous expression of Streptococcus mutans antigen GbpB by L. gasseri, leading to a stimulation of the host immune response.
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