Green Synthesis of Silver Nanoparticles using Bark Extract of Salix Alba and its Antimicrobial Effect Against Bacteria Isolated from Dental Plaque

Now a days oral infections are major concern for the researchers due to chewing large amount of Tabaco, poor hygiene of mouth as well as smoking. These common life styles can enhance the oral infection as well as can play an important role in enhancing the bacterial resistance by the accumulation of antibiotics. Hence my present work was to focused on the biosynthesis of silver nanoparticles using Salix alba bark extract. The color of the plant extract changes in to dark brown upon addition of silver nitrate indicates the formation of silver nanoparticles which completes the reaction after 24 hours.. These nanoparticles were characterized by different microscopic techniques like UV-Vis spectrophotometry which showed the absorption peak at 440 nm specific for silver nanoparticles. HR-TEM showed average size was between 29-35 nm and the tiny particles were seemed to be spherical in morphology. Zeta potential showed silver nanoparticles were stable due to the electrostatic repulsion without adding a different physical or chemical capping agent. Atomic Force Microscopy ( AFM) showed nanoparticles size was 30-50 nm and were stable.. The dental caries forming bacteria were isolated from plaque samples like Lactobacillus sp, Streptococcus sp. and Staphylococcus sp. and compare with different reference strains. Antibacterial activity of these biologically synthesized silver nanoparticles was evaluated by disc diffusion. These synthesized silver nanoparticles showed a good antibacterial activity against the bacteria isolates.. Furthermore these nanoparticles showed quite significant activity along with different antibiotics like Amoxicillin, Tetracycline and Ciprofloxacin during the present study.