Journal
BIOSENSORS-BASEL
Volume 6, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/bios6020022
Keywords
ampicillin; flow immunosensor SPR; sandwich method
Funding
- Sapienza University of Rome, Protezione dell'Ambiente e dei Beni Culturali (CIABC) center
- Istituto per lo studio dei Materiali Nanostrutturati (ISMN) of CNR (Consiglio Nazionale delle Ricerche)
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In this study, we report the development of an SPR (Surface Plasmon Resonance) immunosensor for the detection of ampicillin, operating under flow conditions. SPR sensors based on both direct (with the immobilization of the antibody) and competitive (with the immobilization of the antigen) methods did not allow the detection of ampicillin. Therefore, a sandwich-based sensor was developed which showed a good linear response towards ampicillin between 10(-3) and 10(-1) M, a measurement time of <= 20 min and a high selectivity both towards beta-lactam antibiotics and antibiotics of different classes.
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