Journal
ACTA BIOMATERIALIA
Volume 75, Issue -, Pages 213-225Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2018.06.003
Keywords
Tumor invasion; Tumor spheroid; 3D model; Biomaterial; Hydrogel
Funding
- United States National Institutes of Health [R01CA196018, U01CA210152]
- NATIONAL CANCER INSTITUTE [U01CA210152, R01CA196018, R01CA195655] Funding Source: NIH RePORTER
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Extracellular matrix regulates hallmark features of cancer through biochemical and mechanical signals, although mechanistic understanding of these processes remains limited by lack of models that recreate physiology of tumors. To tissue-engineer models that recapitulate three-dimensional architecture and signaling in tumors, there is a pressing need for new materials permitting flexible control of mechanical and biophysical features. We developed a hybrid hydrogel system composed of collagen and alginate to model tumor environments in breast cancer and other malignancies. Material properties of the hydrogel, including stiffness, microstructure and porosimetry, encompass parameters present in normal organs and tumors. The hydrogel possesses a well-organized, homogenous microstructure with adjustable mechanical stiffness and excellent permeability. Upon embedding multicellular tumor spheroids, we constructed a 3D tumor invasion model showing follow-the-leader migration with fibroblasts leading invasion of cancer cells similar to in vivo. We also demonstrated effects of CXCL12-CXCR4 signaling, a pathway implicated in tumor progression and metastasis, in a dual-tumor spheroid invasion model in 3D hydrogels. These studies establish a new hydrogel platform with material properties that can be tuned to investigate effects of environmental conditions on tumor progression, which will advance future studies of cancer cell invasion and response to therapy. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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