Journal
ACS NANO
Volume 12, Issue 6, Pages 5761-5773Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.8b01640
Keywords
nanosensor; acetylcholine; MRI contrast agents; neurotransmitter; biosensors
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Funding
- National Institutes of Health [R01NS081641]
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A suite of imaging tools for detecting specific chemicals in the central nervous system could accelerate the understanding of neural signaling events critical to brain function and disease. Here, we introduce a class of nanoparticle sensors for the highly specific detection of acetylcholine in the living brain using magnetic resonance imaging. The nanosensor is composed of acetylcholine catalyzing enzymes and pH-sensitive gadolinium contrast agents co-localized onto the surface of polymer nano particles, which leads to changes in T-1, relaxation rate (1/T-1). The mechanism of the sensor involves the enzymatic hydrolysis of acetylcholine leading to a localized decrease in pH which is detected by the pH-sensitive gadolinium chelate. The concomitant change in 1/T-1 in vitro measured a 20% increase from 0 to 10 acetylcholine concentration. The applicability of the nanosensors in vivo was demonstrated in the rat medial prefrontal cortex showing distinct changes in 1/T-1, induced by pharmacological stimuli. The highly specific acetylcholine nanosensor we present here offers a promising strategy for detection of cholinergic neurotransmission and will facilitate our understanding of brain function through chemical imaging.
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