Journal
ACS CHEMICAL NEUROSCIENCE
Volume 9, Issue 8, Pages 1975-1985Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acschemneuro.8b00098
Keywords
Tissue culture; brain slices; primary cell culture; model systems; neurons; astrocytes
Funding
- Swiss National Science Foundation [31003A-179399]
- Swiss National Science Foundation (SNF) [31003A_179399] Funding Source: Swiss National Science Foundation (SNF)
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To investigate the enormous complexity of the functional and pathological brain there are a number of possible experimental model systems to choose from. Depending on the research question choosing the appropriate model may not be a trivial task, and given the dynamic and intricate nature of an intact living brain several models might be needed to properly address certain questions. In this review, we aim to provide an overview of neural cell and tissue culture, reflecting on historic methodological milestones and providing a brief overview of the state-of-the-art. We additionally present an example of an effective model system pipeline, composed of dissociated mouse cultures, organotypics, acute mouse order. The sequential use of these four model systems allows a balance and progression from experimental control to human applicability, and provides a meta-model that can help validate basic research findings in a translational setting. We then conclude with a few remarks regarding the necessity of an integrated approach when performing translational and neuropharmacological studies.
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