Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 28, Pages 23700-23710Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b08606
Keywords
bone targeting; gene delivery; stem cell homing; SDF-1; osteoporosis therapy
Funding
- National Natural Science Foundation of China [81672226]
- Science and Technology Research Funding of Shenzhen [JCYJ20160531174634936, JCYJ20170413162540673]
- Shenzhen Peacock Program [110811003586331]
- Science and technology project of Guangdong [2016A020222007]
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Stem cells are well-known for their great capacity for tissue regeneration. This provides a promising source for cell-based therapies in treating various bone degenerative disorders. However, the major hurdles for their application in transplantation are the poor bone marrow homing and engraftment efficiencies. Stromal cell-derived factor 1 (SDF-1) has been identified as a major stem cell homing factor. With the aims of bone targeted SDF-1 delivery and regulating MSCs migration, alendronate modified liposomal nanoparticles (Aln-Lipo) carrying SDF-1 gene were developed in this study. Alendronate modification significantly increased the mineral binding affinity of liposomes, and facilitated the gene delivery to osteoblastic cells. Up-regulated SDF-1 expression in osteoblasts triggered MSCs migration. Systemic infusion of Aln-Lipo-SDF-1 with fluorescence labeling in mice showed the accumulation in osseous tissue by biophotonic imaging. Corresponding to the delivered SDF-1, the transplanted GFP+ MSCs were attracted to bone marrow and contributed to bone regeneration. This study may provide a useful technique in regulating stem cell migration.
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