Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 10, Pages 8426-8435Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b14888
Keywords
electrospinning; heparin; cationic amphiphilic copolymer; nanofiber yarn; vascular suture
Funding
- National Science Foundation [ECC-1542101]
- University of Washington
- Molecular Engineering & Sciences Institute
- Clean Energy Institute
- National Institutes of Health
- National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH) through the SCBioCRAFT COBRE Center of Regeneration and Formation of Tissues [5P20GM103444-07]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM103444] Funding Source: NIH RePORTER
Ask authors/readers for more resources
The surgical connection of blood vessels, anastomosis, is a critical procedure in many reparative, transplantation, and reconstructive surgical procedures. However, effective restoration of circulation is complicated by pathological clotting (thrombosis) or progressive occlusion due to excess cell proliferation that often leads to additional surgeries and increases morbidity and mortality risk for patients. Pharmaceutical agents have been tested to prevent these complications, but many have unacceptable systemic side effects. Therefore, an alternative approach to deliver these drugs at the site of injury in a controlled manner is necessary. The objective of this study was to develop electrospun nanofibers composed of polyester poly(lactide-co-glycolide) (PLGA), poly(ethylene oxide) (PEO), and positively charged copolymer, poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) for electrostatic binding and release of heparin for application as an antithrombotic microvascular suture. PgP was synthesized with different coupling ratios between PLGA and branched polyethylenimine (bPEI) to obtain PgP(1) (similar to 1 PLGA grafted to 1 bPEI) and PgP(3.7) (similar to 3.7 PLGA grafted to 1 bPEI). Nanofiber yarns (PLGA/PEO/PgP(1) and PLGA/PEO/PgP(3.7)) were fabricated by electrospinning. Heparin immobilization on the positively charged nanofiber yarns was visualized using fluorescein-conjugated heparin (F-Hep), and the amount of immobilized F-Hep was higher on both PLGA/PEO/PgP(3.7) and PLGA/PEO/PgP(1) than yarns without PgP (PLGA/PEO). We also found that F-Hep was released from both PgP-containing yarns in a sustained manner over 20 days, while over 60% of F-Hep was released within 4 h from PLGA/PEO. Finally, we observed that heparin-eluting nanofiber yarns with both PgP(1) and PgP(3.7) showed significantly longer clotting times than nanofiber yarns without PgP. The clotting time of PLGA/PEO/PgP(3.7) was not significantly different than that of free heparin (0.5 mu g/mL). These results show that heparin-eluting electrospun nanofiber yarns may offer a basis for the development of microvascular sutures with anticoagulant activity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available