Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 4, Pages 3305-3313Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b16225
Keywords
cancer cell imaging; hyaluronan; carbon dot; internal light source; molecularly imprinted polymer; glucuronic acid; synthetic antibody
Funding
- Scientific and Technological Research Council of Turkey (TUBITAK) [BIDEB 2214/A]
- Ege University Scientific Research Project [2017 FEN 007]
- Mexican National Council for Science and Technology (CONACYT)
- Institute para el Desarrollo de la Sociedad del Conocimiento del Estado de Aguascalientes (IDSCEA)
- European Regional Development Fund
- Region of Picardy (CPER)
- Region of Picardy (project Polysense)
- European Commission (FP7Marie Curie Actions: SAMOSS) [PITN-2013-607590]
- DFG Research Training Group Electronic Properties of Carbon Based Nanostructures [GRK 1570]
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War against cancer constantly requires new affinity tools to selectively detect, localize, and quantify biomarkers for diagnosis or prognosis. Herein, carbon nanodots (CDs), an emerging class of fluorescent nanomaterials, coupled with molecularly imprinted polymers (MIPs), are employed as a biocompatible optical imaging tool for probing cancer biomarkers. First, N-doped CDs were prepared by hydrothermal synthesis using starch as carbon source and Ltryptophan as nitrogen atom provider to achieve a high quantum yield of 25.1 +/- 2%. The CDs have a typical size of similar to 3.2 nm and produce an intense fluorescence at 450 nm upon excitation with UV light. A MIP shell for specific recognition of glucuronic acid (GlcA) was then synthesized around the CDs, using the emission of the CDs as an internal light source for photopolymerization. GlcA is a substructure (epitope) of hyaluronan, a biomarker for certain cancers. The biotargeting and bioimaging of hyaluronan on fixated human cervical cancer cells using CD core-MIP shell nanocomposites is demonstrated. Human keratinocytes were used as noncancerous reference cells and indeed, less staining was observed by the CD-MIP.
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