Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 14, Pages 11896-11906Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b02423
Keywords
metal-free; porous graphitic carbon; metal-organic framework; glycan; profiling enrichment; size exclusion
Funding
- National Natural Science Foundation of China [21175134, 21375125, 21505134, 21675156]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201503]
- CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry
- Innovation Program of Science and Research from the Dalian Institute of Chemical Physics [DICP TMSR201601]
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In this work, a highly efficient profiling of N-linked glycans was achieved by a facile and eco-friendly synthesized highly porous metal-free carbon material. The metal-free carbon was derived from a well-defined nanorod zinc metal-organic framework via the metal removal under a high-temperature carbonization, which exhibited a highly specific surface area of 1700 m(2)/g. After further oxidation, the oxidized metal-free carbon was applied to the selective isolation of N-linked glycans from complex biological samples due to the strong interaction between carbon and glycan as well as the size-exclusion mechanism. Twenty six N-linked glycans could be identified from the digest of a standard glycoprotein ovalbumin at a concentration of 0.01 mu g/mu L, and the detection limit of glycans could be down to 1 ng/mu L, with 21 N-linked glycans identified. When the mass ratio of the interfering protein bovine serum albumin vs a standard ovalbumin digest is up to 500:1, there were 24 N-glycans confidentially identified. From a real complex sample of a healthy human serum, there were 43 N-linked glycans identified after the enrichment of oxidized metal-free carbon. In a word, the metal-free carbon is opening up new prospect for the high-throughput identification of glycan.
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