Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 18, Pages 15913-15919Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b05038
Keywords
mesoporous luminescence-functionalized MOF; ECL immunosensor; PiDSD; Ru(bpy)(2) (mcpbpy)(2+); MUC1
Funding
- NSFC [21571149]
- NSF of Chongqing [cstc2016jcyjA0231]
- Innovation and Entrepreneurship Project for Chongqing Returned Scholars [cx2017007]
- Selected Science and Technology Foundation for Returned Scholars
- Foundation of State Key Laboratory of Structural Chemistry [20160010]
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In this work, a novel mesoporous luminescence-functionalized metal-organic framework (Ru-PCN-777) with high stability and excellent electrochemiluminescence (ECL) performance was synthesized by imobilizing Ru(bpy)(2) (mcpbpy)(2+) on the Zr-6 cluster of PCN-777 via a strong coordination bond between Zr4+ and -COO-. Consequently, the Ru(bpy)(2) (mcpbpy)(2+) could not only cover the surface of PCN-777 but also graft into the interior of PCN-777, which greatly increased the loading amount of Ru(bpy)(2) (mcpbpy)(2+) and effectively prevented the leaching of the Ru(bpy)(2) (mcpbpy)(2+) resulting in a stable and high ECL response. Considering the above merits, we utilized the mesoporous Ru-PCN-777 to construct an ECL immunosensor to detect mucin 1 (MUC1) based on proximity-induced intramolecular DNA strand displacement (PiDSD). The ECL signal was further enhanced by the enzyme-assisted DNA recycling amplification strategy. As expected, the immunosensor had excellent sensitivity, specificity, and responded wide linearly to the concentration of MUC1 from 100 fg/mL to 100 ng/mL with a low detection limit of 33.3 fg/mL (S/N = 3). It is the first time that mesoporous Zr-MOF was introduced into ECL system to assay biomolecules, which might expand the application of mesoporous metal-organic frameworks (MOFs) in bioanalysis. This work indicates that the use of highly stable mesoporous luminescence-functionalized MOFs to enhance the ECL intensity and stability is a feasible strategy for designing and constructing high-performance ECL materials, and therefore may shed light on new ways to develop highly sensitive and selective ECL sensors.
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