Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 18, Pages 15388-15394Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b01063
Keywords
surface-enhanced Raman scattering (SERS); quantitative detection; hot spot assembly; dual-molecule recognition; dopamine
Funding
- National Science Foundation of China [21605025, 21175028, 21375022]
- Interdisciplinary (Engineering-Medical) Research Fund of Shanghai Jiao Tong University [YG2017QN36]
- Children's Hospital of Shanghai, Shanghai Jiao Tong University
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Reliable profiling of the extracellular dopamine (DA) concentration in the central nervous system is essential for a deep understanding of its biological and pathological functions. However, quantitative determination of this neurotransmitter remains a challenge because of the extremely low concentration of DA in the cerebrospinal fluid (CSF) of patients. Herein, on the basis of the specific recognition of boronate toward diol and N-hydroxysuccinimide ester toward the amine group, a simple and highly sensitive strategy was presented for DA detection by using surface-enhanced Raman scattering (SERS) spectroscopy as a signal readout. This was realized by first immobilizing 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) on gold thin film surfaces to capture DA, followed by introducing 3-mercaptophenylboronic acid (3-MPBA)-functionalized silver nanoparticles to generate numerous plasmonic hot spots with the nanoparticle-on-mirror geometry. Such a dual-recognition mechanism not only avoids complicated bioelement-based manipulations but also efficiently decreases the background signal. With the direct use of the recognition probe 3-MPBA as a Raman reporter, the signal-on SERS method was employed to quantify the concentration of DA from 1 pM to 1 mu M with a detection limit of 0.3 pM. Moreover, our dual-recognition-directed SERS assay exhibited a high resistance to cerebral interference and was successfully applied to monitoring of DA in CSF samples of patients.
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