Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 21, Pages 18372-18379Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b04522
Keywords
polymeric hydrogels; reactive oxygen species; hydrogen peroxide; angiogenesis; tissue regeneration
Funding
- National Research Foundation (NRF)
- Korean government (MSIT) [NRF-2018R1A2B2004529]
- Incheon National University International Cooperative Research Grants in 2015
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Reactive oxygen species (ROS) have been implicated as a critical modulator for various therapeutic applications such as treatment of vascular disorders, wound healing, and cancer treatment. Specifically, growing evidence has recently demonstrated that transient or low levels of hydrogen peroxide (H2O2) facilitates tissue regeneration and wound repair through acute oxidative stress that can evaluate intracellular ROS levels in cells or tissues. Herein, we report a gelatin-based H2O2-releasing hydrogel formed by dual enzyme-mediated reaction using horseradish peroxidase and glucose oxidase (GO(x)). The release behavior of H2O2 from the hydrogel matrices can be precisely controlled by varying the GO(x) concentrations. We demonstrate that H2O2-releasing hydrogels with the optimal condition increase transient upregulation of intracellular ROS levels in the endothelial cells (ECs), enhance proliferative activities of ECs in vitro, and facilitate neovascularization in ovo. We suggest that our H2O2-releasing hydrogels hold great potential as an injectable and dynamic matrix for the treatment of vascular disorders as well as in tissue regenerative medicine.
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