Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 6, Pages 5909-5917Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b18999
Keywords
pH-Responsk; Cyclodextrin vesicle; Monolith; Myoglobin; Mass spectrometry
Funding
- National Natural Science Foundation of China [21575049]
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beta-Cyclodextrin vesicles (CDVs) were first introduced into the polymer monolith to prepare a pH-responsive adsorption material and used for capture and release of a cardiac biomarker, myoglobin (Myo). SH-CDV was decorated with adamantane-modified SH-octapeptide to enhance the encapsulation and release rates of Myo. Afterward, SH-CDV was introduced into the polymer monolith via click reaction to produce a pH-responsive monolith. Combining with the mass spectrometry detection, the CDV-based pH-responsive monolith was used for the enrichment of Myo glycopeptides from the mixture of glycopeptides and nonglycoprotein (bovine serum albumin) tryptsin digests reach up to 1:10 000. A limit of detection of 0.1 fmol was obtained for Myo glycopeptides in the blood sample, indicating the high sensitivity of the method. The prepared CDV-based hybrid monolith demonstrated itself to be a promising material for capture of glycoproteins in complex samples, which provides an efficient strategy for the identification and discovery of biomarkers of acute myocardial infarction.
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