Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 17, Pages 15200-15206Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.7b19717
Keywords
prostate-specific antigen (PSA); polyacrylamide gel-contained zinc finger peptide (PZF); surface-enhanced Raman scattering (SERS); zinc finger peptide-Zn2+ recognition; sandwich immunoassay
Funding
- NNSF of China [21775124, 21575116, 21675129]
- Fundamental Research Funds for the Central Universities, China [XDJK2015C099, SWU114079]
- China Postdoctoral Science Foundation [2015M572427]
- Chongqing Postdoctoral Research Project [xm2015019]
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In this work, we adopted polyacrylamide gel-contained zinc finger peptide (PZF) as a lock of Raman signal and zinc ions (Zn2+) as a sensitive key, which was converted from target-captured ZnO NPs, to achieve the measurement of prostate-specific antigen (PSA). Owing to the lock effect from PZF, the surface-enhanced Raman scattering (SERS) tag toluidine blue (TB) connected on Ag NP-coating silica wafer was sheltered leading to low Raman response. Meanwhile, target PSA can specifically connect with antibody 2-coupled ZnO nanocomplexes (ZnO@Au@Ab(2)) and antibody 1-coupled magnetic (CoFe2O4@Au@Ab(1)) nanocomposite through sandwich immunoassay. In the presence of HCI, the ZnO NPs would convert into Zn2+ to open the PZF because Zn2+ can specifically react with zinc finger peptide to destroy the PZF structure forming abundant pores. In this way, Zn2+ could act as the key of Raman signal to open the PZF structure obtaining a strong Raman signal of TB. The proposed SERS sensor can have a quantitative detection of PSA within the range of 1 pg mL(-1) to 10 ng mL(-1) with a detection limit of 0.65 pg mL(-1). The interaction between zinc finger peptide and Zn2+ was firstly applied in SERS sensor for the sensitive detection of PSA These results demonstrated that the new designed SERS biosensor could be a promising tool in biomarker diagnosis.
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