Journal
NATURE BIOMEDICAL ENGINEERING
Volume 1, Issue 3, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41551-017-0039
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Funding
- National Institutes of Health [R01GM101420-01A1]
- Swiss National Science Foundation through the advanced postdoc mobility fellowship [P300P3_151179]
- Keith Murdoch Fellowship via the American Australian Association
- Life Sciences Research Foundation Fellowship - Good Ventures
- Broadnext10 Catalytic Steps from Broad Institute
- Swiss National Science Foundation (SNF) [P300P3_151179] Funding Source: Swiss National Science Foundation (SNF)
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Nuclear transfection of DNA into mammalian cells is challenging yet critical for many biological and medical studies. Here, by combining cell squeezing and electric-field-driven transport in a device that integrates microfluidic channels with constrictions and microelectrodes, we demonstrate nuclear delivery of plasmid DNA within 1 h after treatment-the most rapid DNA expression in a high-throughput setting (up to millions of cells per minute per device). Passing cells at high speed through microfluidic constrictions smaller than the cell diameter mechanically disrupts the cell membrane, allowing a subsequent electric field to further disrupt the nuclear envelope and drive DNA molecules into the cytoplasm and nucleus. By tracking the localization of the endosomal sorting complex required for transport III protein CHMP4B (charged multivesicular body protein 4B), we show that the integrity of the nuclear envelope is recovered within 15 minutes of treatment. We also provide insight into subcellular delivery by comparing the performance of the disruption-and-field-enhanced method with those of conventional chemical, electroporation and manual-injection systems.
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