Journal
NATURE BIOMEDICAL ENGINEERING
Volume 1, Issue 10, Pages 826-837Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41551-017-0141-6
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Funding
- Canadian Institute of Health Research [201210MFE-289547]
- National Institutes of Health [1K99HL128906, PCBC_JS_2014/4_01, R01 HL123968, HL128170, R01 HL126527, P01 GM099130]
- National Research Foundation of Korea [2012R1A6A3A03039821]
- Burroughs Wellcome Foundation
- National Research Foundation of Korea [2012R1A6A3A03039821] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Many reprogramming methods can generate human induced pluripotent stem cells (hiPSCs) that closely resemble human embryonic stem cells (hESCs). This has led to assessments of how similar hiPSCs are to hESCs, by evaluating differences in gene expression, epigenetic marks and differentiation potential. However, all previous studies were performed using hiPSCs acquired from different laboratories, passage numbers, culturing conditions, genetic backgrounds and reprogramming methods, all of which may contribute to the reported differences. Here, by using high-throughput sequencing under standardized cell culturing conditions and passage number, we compare the epigenetic signatures (H3K4me3, H3K27me3 and HDAC2 ChIP-seq profiles) and transcriptome differences (by RNA-seq) of hiPSCs generated from the same primary fibroblast population by using six different reprogramming methods. We found that the reprogramming method impacts the resulting transcriptome and that all hiPSC lines could terminally differentiate, regardless of the reprogramming method. Moreover, by comparing the differences between the hiPSC and hESC lines, we observed a significant proportion of differentially expressed genes that could be attributed to polycomb repressive complex targets.
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