Journal
GEROSCIENCE
Volume 39, Issue 4, Pages 465-473Publisher
SPRINGER
DOI: 10.1007/s11357-017-9980-z
Keywords
Neurovascular coupling; Functional hyperemia; Laser speckle contrast imaging; Laser speckle contrast analysis; LASCA; Laser speckle imaging; LSI
Categories
Funding
- American Heart Association
- National Center for Complementary and Alternative Medicine [R01-AT006526]
- National Institute on Aging [R01-AG047879, R01-AG038747]
- NIA [T32AG052363, 3P30AG050911-02S1]
- National Institute of Neurological Disorders and Stroke (NINDS) [R01-NS056218]
- Oklahoma Shared Clinical and Translational Resources [NIGMS U54GM104938]
- Oklahoma Center for the Advancement of Science and Technology
- Reynolds Foundation
- Presbyterian Health Foundation
- Merit Review Award from the US Department of Veterans Affairs [I01 BX002211-01A2]
- William & Ella Owens Medical Research Foundation
- San Antonio Nathan Shock Center of Excellence in the Biology of Aging [2 P30 AG013319-21]
- Robert L. Bailey and daughter Lisa K. Bailey Alzheimer's Fund in memory of Jo Nell Bailey
- National Research, Development and Innovation Office of Hungary [K111923]
- Bolyai Janos Research Scholarship of the Hungarian Academy of Sciences [BO/00327/14/5]
- EU [EFOP-3.6.1-16-2016-00008]
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Increasing evidence from epidemiological, clinical, and experimental studies indicates that cerebromicrovascular dysfunction and microcirculatory damage play critical roles in the pathogenesis of many types of dementia in the elderly, including both vascular cognitive impairment (VCI) and Alzheimer's disease. Vascular contributions to cognitive impairment and dementia (VCID) include impairment of neurovascular coupling responses/functional hyperemia (neurovascular uncoupling). Due to the growing interest in understanding and pharmacologically targeting pathophysiological mechanisms of VCID, there is an increasing need for sensitive, easy-to-establish methods to assess neurovascular coupling responses. Laser speckle contrast imaging (LSCI) is a technique that allows rapid and minimally invasive visualization of changes in regional cerebromicrovascular blood perfusion. This type of imaging technique combines high resolution and speed to provide great spatiotemporal accuracy to measure moment-to-moment changes in cerebral blood flow induced by neuronal activation. Here, we provide detailed protocols for the successful measurement in neurovascular coupling responses in anesthetized mice equipped with a thinned-skull cranial window using LSCI. This method can be used to evaluate the effects of anti-aging or anti-AD treatments on cerebromicrovascular health.
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