Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 7, Issue 32, Pages 17598-17602Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b06298
Keywords
cell isolation; photopolymerization; polymer; coatings; protein expression; sorting
Funding
- NIH [R21 EB012188, R01 HL127682-01]
- National Science Foundation [CBET-1351531, EEC-0851716]
- National Cancer Institute (NCI) [R25CA153954]
- National Cancer Institute Cancer Nanotechnology Training Center (NCI-CNTC) Traineeship
- Directorate For Engineering [1351531] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys [1351531] Funding Source: National Science Foundation
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Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of targeted, protective polymer coatings on cells for the high speed enrichment of cells. Individual, antigen-positive cells are coated with a bio compatible hydrogel which protects the cells from a surfactant solution, while uncoated cells are immediately lysed. After lysis, the polymer coating is removed through orthogonal photochemistry, and the isolate has >50% yield of viable cells and these cells proliferate at rates comparable to control cells. Minority cell populations are enriched from erythrocyte-depleted blood to >99% purity, whereas the entire batch process requires 1 h and <$2000 in equipment. Batch scale-up is only contingent on irradiation area for the coating photopolymerization, as surfactant-based lysis can be easily achieved on any scale.
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