Journal
MICROSYSTEMS & NANOENGINEERING
Volume 3, Issue -, Pages 1-7Publisher
SPRINGERNATURE
DOI: 10.1038/micronano.2016.80
Keywords
cardiomyocytes; drug screening; electrophysiology; human pluripotent stem cells; multielectrode array
Funding
- NSF [1055112]
- NIH [NS082125, HL133272, HL126527, HL128170, K99/R00 HL121177, 16BGIA27790017]
- National Science Foundation Graduate Fellowship
- Stanford Graduate Fellowship
- Stanford Bio-X Graduate Fellowship
- Packard Fellowship
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1055112] Funding Source: National Science Foundation
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The measurement of the electrophysiology of human pluripotent stem cell-derived cardiomyocytes is critical for their biomedical applications, from disease modeling to drug screening. Yet, a method that enables the high-throughput intracellular electrophysiology measurement of single cardiomyocytes in adherent culture is not available. To address this area, we have fabricated vertical nanopillar electrodes that can record intracellular action potentials from up to 60 single beating cardiomyocytes. Intracellular access is achieved by highly localized electroporation, which allows for low impedance electrical access to the intracellular voltage. Herein, we demonstrate that this method provides the accurate measurement of the shape and duration of intracellular action potentials, validated by patch clamp, and can facilitate cellular drug screening and disease modeling using human pluripotent stem cells. This study validates the use of nanopillar electrodes for myriad further applications of human pluripotent stem cell-derived cardiomyocytes such as cardiomyocyte maturation monitoring and electrophysiology-contractile force correlation.
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