Journal
ACTA NEUROPATHOLOGICA COMMUNICATIONS
Volume 5, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s40478-017-0413-0
Keywords
alpha-synuclein; Parkinson; Prion; Marmoset; Circuits
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology [JP26117005]
- Japan Society for the Promotion of Science [JP23228004]
- Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from the Japan Agency for Medical Research and Development (AMED) [JP14533254]
- Grants-in-Aid for Scientific Research [16H05324] Funding Source: KAKEN
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alpha-Synuclein is a defining, key component of Lewy bodies and Lewy neurites in Parkinson's disease (PD) and dementia with Lewy bodies (DLB), as well as glial cytoplasmic inclusions in multiple system atrophy (MSA). The distribution and spreading of these pathologies are closely correlated with disease progression. Recent studies have revealed that intracerebral injection of synthetic alpha-synuclein fibrils or pathological alpha-synuclein prepared from DLB or MSA brains into wild-type or transgenic animal brains induced prion-like propagation of phosphorylated alpha-synuclein pathology. The common marmoset is a very small primate that is expected to be a useful model of human diseases. Here, we show that intracerebral injection of synthetic alpha-synuclein fibrils into adult wild-type marmoset brains (caudate nucleus and/or putamen) resulted in spreading of abundant alpha-synuclein pathologies, which were positive for various antibodies to alpha-synuclein, including phospho Ser129-specific antibody, anti-ubiquitin and anti-p62 antibodies, at three months after injection. Remarkably, robust Lewy body-like inclusions were formed in tyrosine hydroxylase (TH)-positive neurons in these marmosets, strongly suggesting the retrograde spreading of abnormal alpha-synuclein from striatum to substantia nigra. Moreover, a significant decrease in the numbers of TH-positive neurons was observed in the injection-side of the brain, where a-synuclein inclusions were deposited. Furthermore, most of the alpha-synuclein inclusions were positive for 1-fluoro-2,5-bis (3-carboxy-4-hydroxystyryl) benzene (FSB) and thioflavin-S, which are dyes widely used to visualize the presence of amyloid. Thus, injection of synthetic alpha-synuclein fibrils into brains of non-transgenic primates induced PD-like alpha-synuclein pathologies within only 3 months after injection. Finally, we provide evidence indicating that neurons with abnormal alpha-synuclein inclusions may be cleared by microglial cells. This is the first marmoset model for alpha-synuclein propagation. It should be helpful in studies to elucidate mechanisms of disease progression and in development and evaluation of disease-modifying drugs for alpha-synucleinopathies.
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