4.7 Article

CXCL14 Acts as a Specific Carrier of CpG DNA into Dendritic Cells and Activates Toll-like Receptor 9-mediated Adaptive Immunity

Journal

EBIOMEDICINE
Volume 24, Issue -, Pages 247-256

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.ebiom.2017.09.012

Keywords

CXCL14; TLR9; CpG DNA; Dendritic cells

Funding

  1. JSPS KAKENHI [JP25860304, JP23390256, JP16H02611]
  2. Takeda Science Foundation
  3. Grants-in-Aid for Scientific Research [17J03475, 16H02611, 16H04728] Funding Source: KAKEN

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CXCL14 is a primordial chemokine that plays multiple roles in tumor suppression, autoimmune arthritis, and obesity-associated insulin resistance. However, the underlying molecular mechanisms are unclear. Here, we show that CXCL14 transports various types of CpG oligodeoxynucleotide (ODN) into the endosomes and lysosomes of bone marrow-derived dendritic cells (DCs), thereby activating Toll-like receptor 9 (TLR9). A combination of CpG ODN (ODN2395) plus CXCL14 induced robust production of IL-12 p40 by wild-type, but not Tlr9knockout, DCs. Consistent with this, ODN2395-mediated activation of DCs was significantly attenuated in Cxcl14-knockout mice. CXCL14 bound CpG ODN with high affinity at pH 7.5, but not at pH 6.0, thereby enabling efficient delivery of CpG ODN to TLR9 in the endosome/lysosome. Furthermore, the CXCL14-CpG ODN complex specifically bound to high affinity CXCL14 receptors on DCs. Thus, CXCL14 serves as a specific carrier of CpG DNA to sensitize TLR9-mediated immunosurveillance. (C) 2017 The Authors. Published by Elsevier B. V. This is an open access article under the CC BY-NC-ND license.

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