Journal
NATURE MICROBIOLOGY
Volume 2, Issue 5, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmicrobiol.2017.5
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Funding
- European Research Council (ERC) Advanced Grant BacCellEpi [670823]
- ANR BACNET [BACNET 10-BINF-02-01]
- ANR Investissement d'Avenir Programme [10-LABX-62-IBEID]
- Human Frontier Science Program (HFSP) [RGP001/2013]
- ERANET Infect-ERA PROANTILIS [ANR-13-IFEC-0004-02]
- Fondation le Roch les Mousquetaires
- Spanish Ministry of Economy and Competitiveness [BIO2014-55238-R]
- Pasteur-Roux Fellowship
- HFSP long-term fellowship
- EMBO long-term fellowship [ALTF 732-2010]
- Institut Carnot-Pasteur Maladies Infectieuses fellowship
- European Research Council (ERC) [670823] Funding Source: European Research Council (ERC)
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To adapt to changing environments, bacteria have evolved numerous pathways that activate stress response genes. In Gram-positive bacteria, the stressosome, a cytoplasmic complex, relays external cues and activates the sigma B regulon. The stressosome is structurally well-characterized in Bacillus, but how it senses stress remains elusive. Here, we report a genome-wide N-terminomic approach in Listeria that strikingly led to the discovery of 19 internal translation initiation sites and 6 miniproteins, among which one, Prli42, is conserved in Firmicutes. Prli42 is membrane-anchored and interacts with orthologues of Bacillus stressosome components. We reconstituted the Listeria stressosome in vitro and visualized its supramolecular structure by electron microscopy. Analysis of a series of Prli42 mutants demonstrated that Prli42 is important for sigma B activation, bacterial growth following oxidative stress and for survival in macrophages. Taken together, our N-terminonic approach unveiled Prli42 as a long-sought link between stress and the stressosome.
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