Journal
NATURE MICROBIOLOGY
Volume 2, Issue 10, Pages 1403-1414Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41564-017-0007-4
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Funding
- Bill and Melinda Gates Foundation [OPP1040399, OPP1054480]
- National Institutes of Health [R01 AI103058, R01 AI50234, R01 AI110329]
- Australian Research Council [LP120200557]
- Medicines for Malaria Venture
- Northern Portugal Regional Operational Programme (NORTE) under Portugal through European Regional Development Fund (FEDER)
- Bill and Melinda Gates Foundation [OPP1040399] Funding Source: Bill and Melinda Gates Foundation
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Antimalarial compounds with dual therapeutic and transmission-blocking activity are desired as high-value partners for combination therapies. Here, we report the identification and characterization of hexahydroquinolines (HHQs) that show low nanomolar potency against both pathogenic and transmissible intra-erythrocytic forms of the malaria parasite Plasmodium falciparum. This activity translates into potent transmission-blocking potential, as shown by in vitro male gamete formation assays and reduced oocyst infection and prevalence in Anopheles mosquitoes. In vivo studies illustrated the ability of lead HHQs to suppress Plasmodium berghei blood-stage parasite proliferation. Resistance selection studies, confirmed by CRISPR-Cas9-based gene editing, identified the digestive vacuole membrane-spanning transporter PfMDR1 (P. falciparum multidrug resistance gene-1) as a determinant of parasite resistance to HHQs. Haemoglobin and haem fractionation assays suggest a mode of action that results in reduced haemozoin levels and might involve inhibition of host haemoglobin uptake into intra-erythrocytic parasites. Furthermore, parasites resistant to HHQs displayed increased susceptibility to several first-line antimalarial drugs, including lumefantrine, confirming that HHQs have a different mode of action to other antimalarials drugs for which PfMDR1 is known to confer resistance. This work evokes therapeutic strategies that combine opposing selective pressures on this parasite transporter as an approach to countering the emergence and transmission of multidrug-resistant P. falciparum malaria.
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