Journal
CHEMISTRYSELECT
Volume 2, Issue 7, Pages 2335-2340Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/slct.201700359
Keywords
Aptamer; DNA; 2 '-O-methyl; RNA; Stability; Serum
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Funding
- NIGMS grant Lauri Strauss Leukemia Foundation [5SC3GM105578-04]
- NSF CBET [40F68-02]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1605904] Funding Source: National Science Foundation
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Nucleic acid aptamers (NAAs) are short synthetic DNA or RNA molecules that specifically fold into distinct three-dimensional structures able to specifically recognize a target. While NAAs show unprecedented promise in a variety of applications, including sensing, therapeutics and diagnostics, one major limitation involves the lack of stability towards omnipresent nucleases. Therefore, we herein report a systematic truncation and incorporation of 2'-O-methyl bases to a DNA aptamer, which results in increased stability without affecting affinity. One of the newly designed analogues is stable up to 24 hours, demonstrating that 2'-O-methyl RNA is an attractive modification to DNA aptamers, especially when theranostic applications are intended.
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