Enantioseparation of Flavanoids, Isoxazolines, Dansyl Amino Acids and β-Blockers on Native and Phenylcarbamoylated α, β and γ-Cyclodextrin Chiral Stationary Phases

In this study, a series of native and p-methylphenylcarbamoylated cyclodextrin chiral stationary phases (CD-CSPs) were developed based on alpha, beta and gamma-cyclodextrin via ether linkage. The as-prepared CSPs were characterized by FTIR, solid-state C-13 NMR, thermogravimetric analysis and elemental analysis. Their enantioseparation properties were comprehensively investigated by using high performance liquid chromatography (HPLC) with various racemates including flavanoids, isoxazolines, dansyl amino acids and beta-blockers. The effects of CD cavity size and CD functionalities on the enantioseparation results were evaluated in detail. The correlation between analyte structures and their chiral resolution were also investigated. A reference chart has been proposed for quick choice of CD-CSPs for enantioseparation of specific racemates.