Journal
CHEMISTRYSELECT
Volume 2, Issue 30, Pages 9810-9814Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/slct.201702322
Keywords
azobenzene; nucleic acids; photocontrol; siRNA
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Funding
- National Sciences and Engineering Research Council
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Fine-tuning the activity of short-interfering RNAs (siRNAs) with light could help overcome several obstacles related to potency, delivery, and off-target effects. In this study, we chemically modify siRNAs that contain azobenzene derivative spacers within the sense strand. These molecules are called siRNAzos and they are successfully accommodated within the RNAi pathway as measured by gene-silencing dose-dependent knockdown. In addition to its RNAi biocompatibility, we are able to photochemically control the activity of the siRNAzos that contain the azobenzene within the central region of the sense strand. We demonstrate it is possible to both inactivate and reactivate several siRNAzos with ultraviolet and visible light, respectively.
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