Biomimicking Oxidative Bromination and DNA Nuclease Activities of a New Structurally Characterised Oxido-Diperoxidomolybdenum(VI) Complex

A new oxido-diperoxido molybdenum complex, [MoO(O-2)(2)(AA)] PPh4 (AA= anthranilic acid) has been designed and synthesized. The Structural characterisation suggests that the molybdenum centre is heptacoordinated with a slight distorted pentagonal bipyramidal geometry. The pentagonal plane is completed with four oxygen atoms from two peroxido moiety and one oxygen atom from the ligand carboxylate group. Among the two axial positions one is occupied by an oxygen atom of the oxido group and the other is occupied by an oxygen atom from the ligand carboxylate moiety. The biomimicking multifunctionality of this molybdenum(VI) complex has been exhibited by the application of it as a catalyst in peroxidative bromination, whereas the nuclease activity has been established by DNA cleavage. The catalytic studies suggest that the present compound is a good biological peroxidase mimic. The DNA binding of the molybdenum complex has been studied by various physicochemical tools like UV-vis, fluorescence, viscosity. The intrinsic binding constant of the complex with DNA is calculated to be (8.14 +/- 0.03) x10(3) M-1. All the physicochemical studies and the viscosity measurements reveal that the complex is a groove binder to CT-DNA. The extent of ability of the complex to induce DNA cleavage was studied by gel electrophoresis techniques. The complex has been found to exhibit cleavage of pUC19 plasmid DNA with good efficiency.