Journal
APPLIED BIOLOGICAL CHEMISTRY
Volume 60, Issue 5, Pages 553-561Publisher
KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
DOI: 10.1007/s13765-017-0311-y
Keywords
Caco-2; Cinnamon; Co-culture; Gut barrier integrity; Gut inflammation
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Funding
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) - Ministry of Agriculture, Food and Rural Affairs (MAFRA) [116012-3]
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Cinnamon is the bark of Cinnamomum japonicum SIEB, which possesses anti-microbial, anti-tyrosinase, and antioxidant activities. However, studies on the intestinal health benefits of cinnamon are scarce. Therefore, we were aimed at assessing the potential anti-inflammatory action of cinnamon extracts in a cellular model of intestinal inflammation. Cinnamon extracts were prepared using hot water extract and 70% ethanol extract. Prior to inducing inflammation, co-cultured Caco-2 cells with RAW264.7 cells were treated with the cinnamon extracts. Lipopolysaccharide (LPS) was treated to induce inflammation in RAW264.7 cells. Compared to LPS-treated groups, the cells treated with cinnamon extracts maintained high transepithelial electrical resistance and decreased tight junction permeability, similar to that shown by control Caco-2 cells. In addition, cinnamon extract-treated cells showed decreased levels of inflammatory cytokines, namely nitrite (NO), PGE(2), interleukin (IL)-6, IL-8, and TNF-alpha. These results show that cinnamon may serve as a probable agent for the development of functional supplements for maintenance of gastrointestinal health.
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