Journal
ACS SENSORS
Volume 2, Issue 2, Pages 290-299Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00823
Keywords
multiconstriction channel; microfluidic cell separation; breast cancer cells; smartphone imaging; velocity profiles; video/image processing; particle tracking
Funding
- National Institute of Health (NIH) National Cancer Institute [R21CA210126]
- National Science Foundation (NSF) [CBET-1403304]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1403304] Funding Source: National Science Foundation
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A microfluidic device composed of variable numbers of multiconstriction channels is reported in this paper to differentiate a human breast cancer cell line, MDAMB-231, and a nontumorigenic human breast cell line, MCF10A. Differences between their mechanical properties were assessed by comparing the effect of single or multiple relaxations on their velocity profiles which is a novel measure of their deformation ability. Videos of the cells were recorded via a microscope using a smartphone, and imported to a tracking software to gain the position information on the cells. Our results indicated that a multiconstriction channel design with five deformation (50 mu m in length, 10 mu m in width, and 8 mu m in height) separated by four relaxation (50 mu m in length, 40 mu m in width, and 30 mu m in height) regions was superior to a single deformation design in differentiating MDA-MB-231 and MCF-10A cells. Velocity profile criteria can achieve a differentiation accuracy around 95% for both MDA-MB-231 and MCF-10A cells.
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