Journal
ACS SENSORS
Volume 2, Issue 3, Pages 436-442Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.7b00035
Keywords
lysosome; pH changes; ratiometric fluorescence imaging; autophagy; cell death; viral infection
Funding
- 973 program [2013CB933901]
- NSF China [21572189, 212721, 9691429301, 31420103910, 31330047, 31221065]
- Fundamental Research Funds for the Central Universities [20720160052, 20720150047]
- National Scientific and Technological Major Project [2013ZX10002-002]
- Hi-Tech Research and Development Program of China (863program) [2012AA02A201]
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The organelle-specific pH is crucial for cell homeostasis. Aberrant pH of lysosomes has been manifested in myriad diseases. To probe lysosome responses to cell stress, we herein report the detection of lysosomal pH changes with a dual colored probe (CM-ROX), featuring a coumarin domain with '' always-on '' blue fluorescence and a rhodamine lactam domain activatable to lysosomal acidity to give red fluorescence. With sensitive ratiometric signals upon subtle pH changes, CM-ROX enables discernment of lysosomal pH changes in cells undergoing autophagy, cell death, and viral infection.
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