Journal
ACS SENSORS
Volume 2, Issue 9, Pages 1257-1261Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.7b00425
Keywords
glutathione; mitochondria; oxidative stress; sensors and probes; fluorescence imaging; flow cytometry
Funding
- National Institutes of Health [R01-GM115622, ROI-CA207701, R21-CA213535, R01-AG045183, R01-AT009050, R21-EB022302, DP1-DK113644]
- Welch Foundation [Q1912]
- Cancer Prevention and Research Institute of Texas (CPRIT ) [R1104]
- IDDRC Microscopy Core [P30HD024064, 1U54 HD083092]
- Optical Imaging and Vital Microscopy core
- Cytometry and Cell Sorting Core at Baylor College of Medicine
- NIH [A1036211, CA125123, RR024574, DK56338]
- Integrated Microscopy Core at Baylor College of Medicine
- CPRIT [RP150578]
- Dan L. Duncan Comprehensive Cancer Center
- John S. Dunn Gulf Coast Consortium for Chemical Genomics
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We report a mitochondria-specific glutathione (GSH) probe designated as Mito-RealThiol (MitoRT) that can monitor in vivo real-time mitochondria] glutathione dynamics, and apply this probe to follow mitochondrial GSH dynamic changes in living cells for the first time. MitoRT can be utilized in confocal microscopy, super-resolution fluorescence imaging, and flow cytometry systems. Using MitoRT, we demonstrate that cells have a high priority to maintain the GSH level in mitochondria compared to the cytosol not only under normal growing conditions but also upon oxidative stress.
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