Preparation, optimization and characterization of chitosan polymer from shrimp shells

Preparation, optimization and characterization of chitosan polymer from shrimp shellsBachir Ben Seghir, M. H. BenhamzaAbstract In this study, chitosan is extracted from shrimp Parapenaeus longirostris shells by a chemical process involving demineralization, deproteinization, decolorization and deacetylation. The effect of chitosan degree of deacetylation DD% is investigated using a model of response surface methodology (RSM). The using of ANOVA regression equation gives a determination coefficient R-2 = 0.9853, indicating the precision of the predicted model. Chitosan derived from chitin treated under optimized conditions of 28.6% of NaOH concentration, a temperature of 81.15 degrees C and a reaction time of 9.55 h, showed the highest value of DD% exceeding 98%. The obtained Chitosan characterization results using a spectral analysis infrared (FTIR), Differential Scanning Calorimetry (DSC), X-ray spectroscopy (DRX) and Scanning electron microscopy (SEM) confirmed the purity of chitosan.