Journal
3 BIOTECH
Volume 8, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-017-1064-3
Keywords
Wheat; Transcription factor; WRKY; Leaf rust; Abiotic stress
Categories
Funding
- BTIS Net Sub DIC [BT/BI/04/065/04]
- CoE-TEQIP-II [NPIU/TEQIP II/FIN/31/158]
- Department of Science and Technology INSPIRE [2011/318]
- Council of Scientific and Industrial Research [9/554 (0026) 2010-EMR-I]
Ask authors/readers for more resources
WRKY, a plant-specific transcription factor family, plays vital roles in pathogen defense, abiotic stress, and phytohormone signalling. Little is known about the roles and function of WRKY transcription factors in response to rust diseases in wheat. In the present study, three TaWRKY genes encoding complete protein sequences were cloned. They belonged to class II and III WRKY based on the number of WRKY domains and the pattern of zinc finger structures. Twenty-two DNA-protein binding docking complexes predicted stable interactions of WRKY domain with W-box. Quantitative real-time-PCR using wheat near-isogenic lines with or without Lr28 gene revealed differential up-or down-regulation in response to biotic and abiotic stress treatments which could be responsible for their functional divergence in wheat. TaWRKY62 was found to be induced upon treatment with JA, MJ, and SA and reduced after ABA treatments. Maximum induction of six out of seven genes occurred at 48 h post inoculation due to pathogen inoculation. Hence, TaWRKY (49, 50, 52, 55, 57, and 62) can be considered as potential candidate genes for further functional validation as well as for crop improvement programs for stress resistance. The results of the present study will enhance knowledge towards understanding the molecular basis of mode of action of WRKY transcription factor genes in wheat and their role during leaf rust pathogenesis in particular.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available