Journal
MOLECULAR IMAGING
Volume 16, Issue -, Pages 1-9Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/1536012117704557
Keywords
Alzheimer disease; brown adipose tissue; norepinephrine; brain; PET imaging; transgenic mice 2576; glucose metabolism
Funding
- NIH [AG029479]
Ask authors/readers for more resources
Objective: Imaging animal models of Alzheimer disease (AD) is useful for the development of therapeutic drugs and understanding AD. Transgenic Swedish hAPPswe Tg2576 mice are a good model of beta-amyloid plaques. We report F-18-fluoro-2-deoxyglucose (F-18-FDG) positron emission tomography (PET) imaging of brain and intrascapular brown adipose tissue (IBAT) in transgenic mice 2576 (Tg2576) and wild-type (WT) mice. Methods: Transgenic Tg2576 mice and WTmice, > 18 months were injected intraperitonally with approximate to 25 to 30 MBq F-18-FDG while awake. After 60 minutes, they were anesthetized with isoflurane (2.5%) and imaged with Inveon MicroPET. Select mice were killed, imaged ex vivo, and 20 mu m sections cut for autoradiography. F-18-FDG uptake in brain and IBAT PET and brain auto-radiographs were analyzed. Results: Fasting blood glucose levels averaged 120 mg/dL for WT and 100 mg/dL for Tg2576. Compared to WT, Tg2576 mice exhibited a decrease in SUVglc in the various brain regions. Average reductions in the cerebrum regions were as high as -20%, while changes in cerebellum were -3%. Uptake of F-18-FDG in IBAT decreased by -60% in Tg2576 mice and was found to be significant. Intrascapular brown adipose tissue findings in Tg2576 mice are new and not previously reported. Use of blood glucose for PET data analysis and corpus callosum as reference region for autoradiographic analysis were important to detect change in Tg2576 mice. Conclusion: Our results suggest that F-18-FDG uptake in the Tg2576 mice brain show F-18-FDG deficits only when blood glucose is taken into consideration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available