Journal
MATERIALS & DESIGN
Volume 127, Issue -, Pages 261-271Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.matdes.2017.04.079
Keywords
Polylactic acid (PLA); Hyaluronic acid (HA); Tissue engineering scaffold (TES); Plasma; Macrophages; Cytokines
Categories
Funding
- Russian Science Foundation [6-13-10239]
- Russian Science Foundation [16-13-10239] Funding Source: Russian Science Foundation
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Bioactive polylactic acid based (PLA) scaffolds with hyaluronic acid immobilized on their surface by atmospheric pressure plasma assisted modification method were developed. By using X-ray photoelectron spectroscopy and wettability measurements it was shown that atmospheric pressure plasma treatment leads to the changes in surface chemical composition of the PLA-based scaffolds that resulted in an increased long-term hydrophilicity of the scaffolds surface. Scanning electron microscopy and mechanical studies revealed that the use of plasma for surface activation allows for the non-destructive immobilization of bioactive compounds like hyaluronic acid. The modified PLA-based scaffolds effect on the release of cytokines and matrix metalloproteinases by primary human monocyte-derived macrophages was investigated. The macrophages reaction to the scaffolds was donor-specific, however, the two best materials from immunological point of view were identified-plasma treated PLA-based scaffold and PLA-based scaffold with the least amount of immobilized hyaluronic acid. Both hyaluronic acid attachment and atmospheric pressure plasma treatment enhance PLA-based scaffolds biocompatibility. It was found that supernatants collected after the macrophages coculture with modified PLA-based scaffolds stimulate HUVECs' tube formation. The modified PLA-based scaffolds possess pro-angiogenic activity. Thus, our research offers a high performing method for the creation of polymer-based tissue engineering scaffolds with modified bioactive surface.
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