Journal
ACS CENTRAL SCIENCE
Volume 3, Issue 2, Pages 117-123Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acscentsci.6b00330
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Funding
- Armenise-Harvard Foundation
- Simons Foundation [290358]
- COST action [4]
- EPSRC [EP/H01912X/1]
- NSF [0944139]
- Autonomous Province of Trento
- EPSRC [EP/H01912X/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H01912X/1] Funding Source: researchfish
- Direct For Social, Behav & Economic Scie
- Divn Of Social and Economic Sciences [0944139] Funding Source: National Science Foundation
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Artificial cells capable of both sensing and sending chemical messages to bacteria have yet to be built. Here we show that artificial cells that are able to sense and synthesize quorum signaling molecules can chemically communicate with V. fischeri, V. harveyi, E. coli, and P. aeruginosa. Activity was assessed by fluorescence, luminescence, RT-qPCR, and RNA-seq. Two potential applications for this technology were demonstrated. First, the extent to which artificial cells could imitate natural cells was quantified by a type of cellular Turing test. Artificial cells capable of sensing and in response synthesizing and releasing N-3-(oxohexanoyl)homoserine lactone showed a high degree of likeness to natural V. fischeri under specific test conditions. Second, artificial cells that sensed V. fischeri and in response degraded a quorum signaling molecule of P. aeruginosa (N-(3-oxododecanoyl)homoserine lactone) were constructed, laying the foundation for future technologies that control complex networks of natural cells.
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