Journal
ACTA PHARMACEUTICA
Volume 66, Issue 4, Pages 543-554Publisher
HRVATSKO FARMACEUTSKO DRUSTOV (HFD)-CROATION PHARMACEUTICAL SOC
DOI: 10.1515/acph-2016-0038
Keywords
Allium cepa; Allium ascalonicum; cultivar varieties; flavonols; TLC; quantitative analysis
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This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4'-O-glucoside and quercetin-3,4'-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 ('Exihibition) to 1079 mg kg-1 fresh mass (fm) ('Hybing') in edible parts, and from 1727 ('Hyline') to 28949 mg kg(-1) fm ('Red Baron) in outer scales. The bulbs of two shallot cultivars contained 209 ('Ambition') and 523 mg kg(-1) fm ('Matador') of flavonols in edible parts and 5426 and 8916 mg kg(-1) fm in outer scales, respectively.
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