Journal
MOLECULAR METABOLISM
Volume 6, Issue 8, Pages 797-808Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molmet.2017.05.013
Keywords
Brown adipocytes; Ca2+; Confocal microscopy; Endoplasmic reticulum; Fluorescence imaging; Mitochondria-associated ER membrane
Categories
Funding
- Japan Society for the Promotion of Science (JSPS) KAKENHI [15K05251]
- PRESTO
- Japan Science and Technology Agency [JPMJPR15F5]
- US National Institutes of Health [R01DK077097, R01DK102898]
- Grants-in-Aid for Scientific Research [15K05251] Funding Source: KAKEN
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Objective: Brown adipocytes (BAs) are endowed with a high metabolic capacity for energy expenditure due to their high mitochondria content. While mitochondrial pH is dynamically regulated in response to stimulation and, in return, affects various metabolic processes, how mitochondrial pH is regulated during adrenergic stimulation-induced thermogenesis is unknown. We aimed to reveal the spatial and temporal dynamics of mitochondrial pH in stimulated BAs and the mechanisms behind the dynamic pH changes. Methods: A mitochondrial targeted pH-sensitive protein, mito-pHluorin, was constructed and transfected to BAs. Transfected BAs were stimulated by an adrenergic agonist, isoproterenol. The pH changes in mitochondria were characterized by dual-color imaging with indicators that monitor mitochondrial membrane potential and heat production. The mechanisms of pH changes were studied by examining the involvement of electron transport chain (ETC) activity and Ca2+ profiles in mitochondria and the intracellular Ca2+ store, the endoplasmic reticulum (ER). Results: A triphasic mitochondrial pH change in BAs upon adrenergic stimulation was revealed. In comparison to a thermosensitive dye, we reveal that phases 1 and 2 of the pH increase precede thermogenesis, while phase 3, characterized by a pH decrease, occurs during thermogenesis. The mechanism of pH increase is partially related to ETC. In addition, the pH increase occurs concurrently with an increase in mitochondrial Ca2+. This Ca2+ increase is contributed to by an influx from the ER, and it is further involved in mitochondrial pH regulation. Conclusions: We demonstrate that an increase in mitochondrial pH is implicated as an early event in adrenergically stimulated BAs. We further suggest that this pH increase may play a role in the potentiation of thermogenesis. (C) 2017 The Authors. Published by Elsevier GmbH.
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