PLLA Synthesis and Nanofibers production: Viability by Human Mesenchymal Stem Cell from Adipose Tissue

The absorbable polyacid is one of the most used and studied materials in tissue engineering. This work synthesized a poly (L-lactic acid) (PLLA) through ring-opening polymerization and produced with it nanofibers by the electrospinning process. The PLLA was analyzed by FTIR and its cytotoxicity was evaluated by the MTT assay and Live/Dead (R) (Molecular Probes). The tests were performed in contact with human mesenchymal cells at varying times. The high rates of viability and proliferation of cells in contact with the PLLA shown by MTT and Live/Dead (R) tests demonstrate that this PLLA is a biocompatible material. There was also the successful production of electrospinning nanofibers, which can be converted for specific biomedical applications in the future. (C) 2015 The Authors. Published by Elsevier B.V.