Identification, Molecular Cloning of IL-1β and Its Expression Profile during Nocardia seriolae Infection in Largemouth Bass, Micropterus salmoides

In the present study, IL-1 beta cDNA was identified and analyzed from largemouth bass (Micropterus salmoides). Full length IL-1 beta mRNA was obtained using Rapid Amplification of cDNA Ends (RACE), which contains 78 bp 3'-UTR, a 455 bp 5'-UTR, and an open reading frame (ORF) of 702 bp coding for 233 amino acid residues. The molecular weight and theoretical isoelectric point of largemouth bass IL-1 beta protein was predicted to be 26.7 kDa and 6.08 respectively. A largemouth bass IL-1 beta phylogenetic analysis showed a close relation to the IL-1 beta s of striped trumpeter (Latris lineata), Chinese perch (Siniperca chuatsi), and Japanese sea bass (Lateolabrax japonicus). Peptidoglycan upregulated IL-1 beta in the spleen and head kidney, while lipopolysaccharide upregulated detectable levels of IL-1 beta in the spleen only. Largemouth bass, challenged with Nocardia seriolae (1.0 x 10(6) cfu/mL), showed a significant increase in IL-1 beta at 3 and 5 days post infection (dpi) in the spleen, while in the head kidney significant expression was found at 2 and 3 dpi, peaking at 3 dpi. Furthermore, tumor necrosis factor alpha (TNF-alpha) showed significantly higher expression in the spleen at 3 and 5 dpi, and in the head kidney at 1 and 3 dpi, with expression decreasing at 5 dpi in both tissues.