Journal
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
Volume 11, Issue 4, Pages 529-536Publisher
SPRINGER
DOI: 10.1007/s11705-017-1645-z
Keywords
aptamer; DNA nanoparticles; rolling circle amplification; doxorubicin; drug delivery; pH sensitive
Categories
Funding
- National Key Research and Development Plan [2016YFE0119200]
- National Natural Science Foundation of China [81402856, 81361140344, 21402143]
- Tianjin Municipal Science and Technology Commission [15JCYBJC28700, 15JCQNJC13600]
- National Students' Innovation and Entrepreneurship Training Program [201510062008]
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An anticancer drug delivery system consisting of DNA nanoparticles synthesized by rolling circle amplification (RCA) was developed for prostate cancer membrane antigen (PSMA) targeted cancer therapy. The template of RCA was a DNA oligodeoxynucleotide coded with PSMA-targeted aptamer, drug-loading domain, primer binding site and pH-sensitive spacer. Anticancer drug doxorubicin, as the model drug, was loaded into the drug-loading domain (multiple GC-pair sequences) of the DNA nanoparticles by intercalation. Due to the integrated pH-sensitive spacers in the nanoparticles, in an acidic environment, the cumulative release of doxorubicin was far more than the cumulative release of the drug in the normal physiological environment. In cell uptake experiments, treated with doxorubicin loaded DNA nanoparticles, PSMA-positive C4-2 cells could take up more doxorubicin than PSMA-null PC-3 cells. The prepared DNA nanoparticles showed the potential as drug delivery system for PSMA targeting prostate cancer therapy.
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