Journal
JOURNAL OF BREATH RESEARCH
Volume 10, Issue 4, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1752-7155/10/4/047102
Keywords
Pseudomonas aeruginosa; GC x GC-TOFMS; bacterial infections; biomarkers; comparative metabolomics
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Funding
- Cystic Fibrosis Foundation Pilot and Feasibility Award [Hill16I0]
- Dartmouth Neukom Institute CompX Faculty Grant
- Dartmouth Clinical and Translational Science Institute Synergy Award
- Burroughs Wellcome Fund
- Cystic Fibrosis Foundation Postdoctoral Fellowship [Bean12F0]
- [1014106]
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Pseudomonas aeruginosa is a nearly ubiquitous Gram-negative organism, well known to occupy a multitude of environmental niches and cause human infections at a variety of bodily sites, due to its metabolic flexibility, secondary to extensive genetic heterogeneity at the species level. Because of its dynamic metabolism and clinical importance, we sought to perform a comparative analysis on the volatile metabolome (the 'volatilome') produced by P. aeruginosa clinical isolates. In this study, we analyzed the headspace volatile molecules of 24 P. aeruginosa clinical isolates grown in vitro, using 2D gas chromatography time-of-flight mass spectrometry (GC x GC-TOFMS). We identified 391 non-redundant compounds that we associate with the growth and metabolism of P. aeruginosa (the 'panvolatilome'). Of these, 70 were produced by all 24 isolates (the 'core volatilome'), 52 by only a single isolate, and the remaining 269 volatile molecules by a subset. Sixty-five of the detected compounds could be assigned putative compound identifications, of which 43 had not previously been associated with P. aeruginosa. Using the accessory volatile molecules, we determined the inter-strain variation in the metabolomes of these isolates, clustering strains by their metabotypes. Assessing the extent of metabolomic diversity in P. aeruginosa through an analysis of the volatile molecules that it produces is a critical next step in the identification of novel diagnostic or prognostic biomarkers.
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